Absolute quantification by droplet digital PCR versus analog real-time PCR
نویسندگان
چکیده
منابع مشابه
Droplet volume variability as a critical factor for accuracy of absolute quantification using droplet digital PCR
Accurate and precise nucleic-acid quantification is crucial for clinical and diagnostic decisions, as overestimation or underestimation can lead to misguided treatment of a disease or incorrect labelling of the products. Digital PCR is one of the best tools for absolute nucleic-acid copy-number determination. However, digital PCR needs to be well characterised in terms of accuracy and sources o...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2013
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.2633